CPI require a Raman Spectrometer to be utilised in order to assess the composition of liquid culture samples and to evaluate product quality and titre in an in-line manner. The integrated spectrometer is intended to act as both a primary analytical assay in its own right, and as a robust multiplex alternative to the other cell enumeration, titre and metabolite assays currently available on-site. It is critical to note that the ability of the system to be directly integrated with an ambr-250 at both a hardware and software level is of paramount importance. Raman spectra produced using the spectrometer should be capable of being fed back to the ambr-250 and utilised to control related cell culture parameters, such as feeding rates, in an automated manner. CPV: 38000000, 38000000, 33000000.